RNA Extraction Protocol

Protocol for the extraction of total RNA for use in QPCR and Next Generation Sequencing. Yeilds total RNA that has been DNAse treated and is ready for downstream applications.

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DNA Extraction Protocol

Protocol for the extraction of genomic DNA for use in PCR and Next Generation Sequencing. Yeilds total DNA that has been proteinase and RNase treated and is ready for downstream applications.

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Removing Coral Tissue by Airbrushing

Protocol for the removal of coral tissue from the skeleton using an airbrush. Yields tissue slurry that can be used for biological assays and clean skeleton that can be used for surface area measurement.

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RNA Extraction Protocol

Objective:Test quantity and quality of RNA extracted from fresh coral samples of 3 species (Montipora capitata, Porites compressa, and Pocillopora damicornis). This protocol includes a purification column which has not been used in the previous extractions and should increase quality.

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