RNA Quality Check Protocol

Protocol for checking the quality of RNA. Provides RNA integrity number and microgel to assess degradation and DNA contamination.

RNA 6000 Pico Kit (RNA 6000 Pico Kit, Agilent, Catalog # 5067-1513)

Protocol for Quantification of RNA

  • Heat denature samples at 70°C for 2min and place on ice immediately prior to use
  • Qualitative range 50–5000 pg/μl for Total RNA, and 250–5000 pg/μl mRNA

Procedure

  • Follow Manufacturer’s Instructions exactly
Written on August 26, 2016