Gel Rig Enduro XL Test
Testing the resolution of the Enduro Gel Xl Horizontal Gel Electrophoresis Unit E0160
Equipment and Reagents Labnet E0160]()
- Enduro Gel Xl Labnet E0160
gel samples:112
gel box size:10 x 6.7 x 2.5 in./24.5 x 17 x 6.2 cm
gelsize:- 1 large (12.5 x 12 cm)
- 2 small (12.5 x 6 cm)
- 4 micro (6 x 6 cm)
- Loading dye
- Gel ladder GeneRuler 100 bp DNA Ladder (Thermo FIsher Catalog SM0241)
- Gel ladder GeneRuler 1kb plus DNA Ladder (Thermo FIsher Catalog SM1333)
- Gel Stain Biotium GelGreen Nucleic Acid Gel Stain, 10,000X in Water Fisher Cat NC9728313
Prepare Reagents
- 1x TAE
- 150ml 2% agarose gel in TAE
Gel Rig
Gel Rig Parts 
Gel combs 
Gel Running 
Gel protocol
Prepared a 150ml gel using 150ml of 1xTAE + 3.0g Agarose, melted gel for ~3 minues in the microwave and let cool and added 2µl Gelgreen, mixed and poured in the large gel rig. Used the 14 well comb on top and the 28 well comb on the middel of the gel.
Added 4µl of GeneRuler 1kb plus DNA Ladder already containing dye to gel wells Mixed 1.5µl of Purple loading dye with 4µl of GeneRuler 100 bp DNA Ladder and added to gel wells
Samples
Top row with 14 wells
Top1 - GeneRuler 1kb plus DNA Ladder
Top2 - Empty
Top3 - GeneRuler 100 bp DNA Ladder
Top4 - Empty
Top5 - Empty
Top6 - Empty
Top7 - GeneRuler 1kb plus DNA Ladder
Top8 - Empty
Top9 - GeneRuler 100 bp DNA Ladder
Top10 - Empty
Top11 - Empty
Top12 - GeneRuler 100 bp DNA Ladder
Top13 - Empty
Top14 - GeneRuler 1kb plus DNA Ladder
Bottom row with 28 wells
Bottom1 - GeneRuler 1kb plus DNA Ladder
Bottom2 - Empty
Bottom3 - GeneRuler 100 bp DNA Ladder
Bottom4 - Empty
Bottom5 - Empty
Bottom6 - Empty
Bottom7 - Empty
Bottom8 - Empty
Bottom9 - Empty
Bottom10 - Empty
Bottom11 - Empty
Bottom12 - GeneRuler 100 bp DNA Ladder
Bottom13 - Empty
Bottom14 - Empty
Bottom15 - Empty
Bottom16 - Empty
Bottom17 - GeneRuler 100 bp DNA Ladder
Bottom18 - Empty
Bottom19 - Empty
Bottom20 - Empty
Bottom21 - Empty
Bottom22 - Empty
Bottom23 - Empty
Bottom24 - Empty
Bottom25 - Empty
Bottom26 - GeneRuler 100 bp DNA Ladder
Bottom27 - Empty
Bottom28 - GeneRuler 1kb plus DNA Ladder
Ran the gel in 1X TAE at 70V for 75 minutes
Gel Results
After 40 minutes at 70V
After 70 minutes at 70V
