Protocol for checking the quality of RNA. Provides RNA integrity number and microgel to assess degradation and DNA contamination.

RNA 6000 Pico Kit (RNA 6000 Pico Kit, Agilent, Catalog # 5067-1513)

• Bioanalyzer RNA 6000 Pico Kit Kit Manufacturer’s Instructions
• The Bioanalyzer provides “Quantitation and integrity analysis of total RNA or mRNA, RNA Integrity Number (RIN) – to compare integrity of total RNA samples, and Visualization of rRNA impurities in mRNA samples”

Protocol for Quantification of RNA

• Heat denature samples at 70°C for 2min and place on ice immediately prior to use
• Qualitative range 50–5000 pg/μl for Total RNA, and 250–5000 pg/μl mRNA

Procedure

• Follow Manufacturer’s Instructions exactly